M.Sc Thesis

M.Sc StudentNemcovsky Amar Danielle
SubjectEndothelial Cell Activation in an Embolic Ischemia-
Reperfusion Injury Microfluidic Model
DepartmentDepartment of Biomedical Engineering
Supervisor ASSOCIATE PROF. Netanel Korin
Full Thesis textFull thesis text - English Version


Ischemia, lack of blood supply, is associated with a variety of life-threatening cardiovascular diseases, including acute ischemic stroke and myocardial infraction. While blood flow restoration is critical to prevent further damage, paradoxically, rapid reperfusion can increase tissue damage, this phenomenon is termed Ischemia/Reperfusion injury. One of the first symptoms of IRI is inflammatory response which is accompanied with endothelial cell (EC) activation. Here we present a microfluidic model of IRI lined with human EC, that can be obstructed via a human blood clot and then re-perfused via thrombolytic treatment. After embolic occlusion a significant increase was noticed in the inflammatory receptors, E-selectin and I-CAM1. Following a thrombolytic treatment, flow was restored, and we noted a significant decrease in adherent EC and increase of I-CAM1 expression compared to occluded models. The presented model can be applied to allow better understanding of human embolic based IRI and potentially serve as a platform for the development of improved and new therapeutic approaches.