טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentGhantous Yasmine
SubjectThe Possible Role of Viral Infection in the Etiology of
Oral Cancer
DepartmentDepartment of Medicine
Supervisor Clinical Professor Micha Peled
Full Thesis textFull thesis text - English Version


Abstract

Objective: The aim of the present study is to analyze using Tissue -Array technology and PCR the possible influence of viral infection on the carcinogenesis of oral carcinomas.

Materials and Methods

We included in the study 63 oral neoplasm biopsies.

Immunostaining of formalin fixed, paraffin-embedded 5 microns TissueArray section were performed on sixty-three oral neoplasm biopsies. Slides were deparaffinized and incubated with the primary antibody, LMP1 for EBV, pp65 CMV, and P16 for HPV. The specific intracellular localization is recorded.

DNA from the fresh tissues was extracted according to the manufacturer's protocol (TRIZOL). Primers of specific viral infections were fabricated.

The total DNA was evaluated using PCR (Gene pro, Bioe) As negative control, sterile water was utilized in place of the 2uL DNA sample.

Results

Patients in the experimental group were aged 38 - 92 years (mean 63. years). A predominant number of the OSCC patients had reported tobacco use (47%). A much smaller portion reported consumption of alcohol (6%). immunoreactivity for CMV and EBV was not significant, while, HPV antibody expression was found in 33% of the cases.

Forty percent of the OSCC patients showed expression for HPV DNA in PCR, furthermore The HPV genotype was also expressed in 2 control tissue specimen (9%), one of which had positive margins for OSCC.

Conclusions                                                                                                            

A correlation between HPV and OSCC was found in this study, 40% of the tissue showed expression for HPV DNA. We found a correlation between AM and HPV; as 72% of the were positive for HPV antibody. In this study the role of HPV was demonstrated in oral carcinogenesis, we found that patients who were positive for HPV had better prognosis than patients who were negative for HPV, also we found that the HPV positive patients were younger than the negative patients and with a better over all survival.



Key Words: Human PapillomaVirus, carcinogenesis, Immunohistochemestry, TissueArray, Polymerase Chain Reaction, Oral cancer, Oral squamous cell carcinoma.