M.Sc Thesis

M.Sc StudentElhanan Tal
SubjectMeasuring Blood Velocity uUing Correlative Spectrally
Encoded Flow Cytometry
DepartmentDepartment of Biomedical Engineering
Supervisor ASSOCIATE PROF. Dvir Yelin
Full Thesis textFull thesis text - English Version


Spectrally encoded flow cytometry (SEFC) is a promising technique for imaging blood in the microcirculation. Yet, the dependency of one of the axes in the SEFC images on time prevents the quantification of prominent clinical parameters. Here we address this challenge by presenting a method for measuring blood flow velocity in small vessels. A modified SEFC system was built, using a wedge prism we generated two spectrally encoded illumination lines displaced in the flow direction. Autocorrelation of the acquired images enables extraction of quantitative flow velocity measurements. The method is demonstrated by measuring the velocity of latex beads in vitro, this experiment established the linear connection between the measured and expected velocities. Blood velocity measurements were also demonstrated in vitro.

To measure WBC velocity, which differs from that of RBC in vivo, another technique was developed based on a relationship between the axes incorporates in the WBC images themselves. Measurements of single WBC velocity were demonstrated in vitro and in vivo, yet a rigorous validation experiment is still required to establish this technique. These two methods allow real-time velocity measurements of up to 11.7 mm/s with high spatial resolution, and could be easily integrated into an existing SEFC system.