טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentNehoray Shachar
SubjectThe Involvement of SRBI in Differentiation of Tr1 Cells
in DDM1 Model
DepartmentDepartment of Medicine
Supervisor Professor Nathan Karin


Abstract

The pathogenesis of effector lineages of CD4 T cells that promote the development and progression of various inflammatory autoimmune diseases, including type I diabetes mellitus (T1DM) is regulated by at least two major subsets of regulatory T cells: FOXP3 and FOXP3- T cells. The latter fall into two major subtypes: Those that predominantly secrete IL-10 (Tr1). These experiments were initiated using the type I diabetes (T1DM) model in NOD mice. As for Tr1 cells, several approaches have been suggested to polarize and extend these cells. A major obstacle in studding the in vivo properties of polarized Tr1 cells is the lack of a biomarker that would assist following their fate and migratory properties.

We found that over activation about 10% of CD4 T cells express the cell transmembrane scavenger receptor B-I (SR-BI, in human Cla1), and that upon interacting with SR-BI specific autoantibodies, or a mAb that our lab developed with high specificity to SR-BI/Cla1, they become IL-10 producing Tregs. The current study focuses on exploring their role in T1DM, and in using our new mAb (E12 mAb) for intervention in the pathogenesis of this disease.