טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
Ph.D Thesis
Ph.D StudentSela Yogev
SubjectInteractions between Cell Cycle and Differentiation in
Human Embryonic Stem Cells
DepartmentDepartment of Medicine
Supervisors Professor Emeritus Joseph Itskovitz
Dr. Yoav Soen
Full Thesis textFull thesis text - English Version


Abstract

The cross-talk between cell cycle and differentiation has been demonstrated for several cellular models. Yet, little is known about this cross talk in hESC whose cell cycle regulation is thought to exhibit reduced sensitivity to cell cycle arrest. In this work we investigated the relations between cell cycle phase and differentiation under self renewing conditions and in response to a potent differentiation signal inducer.

While experimentally induced arrest of human embryonic stem cells (hESCs) in G1 has been shown to stimulate differentiation, it remains unclear whether the unperturbed G1 phase in hESCs is causally related to differentiation. We tested the hypothesis that hESC are more prone to differentiation in G1 by employing centrifugal elutriation to isolate and investigate differentiation propensities of hESCs in different phases of their cell cycle, complemented by exploration of retinoic acid induced differentiation in chemically synchronized cells.