|Ph.D Student||Sela Yogev|
|Subject||Interactions between Cell Cycle and Differentiation in|
Human Embryonic Stem Cells
|Department||Department of Medicine||Supervisors||Professor Emeritus Joseph Itskovitz|
|Dr. Yoav Soen|
|Full Thesis text|
The cross-talk between cell cycle and differentiation has been demonstrated for several cellular models. Yet, little is known about this cross talk in hESC whose cell cycle regulation is thought to exhibit reduced sensitivity to cell cycle arrest. In this work we investigated the relations between cell cycle phase and differentiation under self renewing conditions and in response to a potent differentiation signal inducer.
While experimentally induced arrest of human embryonic stem cells (hESCs) in G1 has been shown to stimulate differentiation, it remains unclear whether the unperturbed G1 phase in hESCs is causally related to differentiation. We tested the hypothesis that hESC are more prone to differentiation in G1 by employing centrifugal elutriation to isolate and investigate differentiation propensities of hESCs in different phases of their cell cycle, complemented by exploration of retinoic acid induced differentiation in chemically synchronized cells.