טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentHadid Yareen
SubjectGenomic Diversity among East Indian Populations
DepartmentDepartment of Medicine
Supervisor Professor Karl Skorecki
Full Thesis textFull thesis text - English Version


Abstract

Analysis of Deoxyribonucleic Acid (DNA) sequence variation has emerged as a powerful tool in studying the history and demography of human populations. Of the various genetics markers the analysis of sequence variation in mitochondrial DNA (mtDNA) to address questions related to maternal origins and demographic patterns in various human populations are widely used. The Indian subcontinent occupies a distinctive place due primarily to its geographical, historical, social, cultural, and political features. One of the most interesting populations from India that have seized the attention of our laboratory is the Kuki-Chin-Mizo population inhabiting the northeastern state of Mizoram and Manipur in India. The reasoning for this is there claim to be descendants of one of the ten lost tribes of Israel.

Buccal swab were collected with informed consent from 349 unrelated male belonging to 7 East Indian tribes from Manipur and Mizoram. This part of the world is located at the geographic demarcation of India and the Far East and consequently serves as an important zone of population interface. In this total set of samples we have studied in depth the sequence variation at both the control region (HVS-1, HVS-II) and coding region of mtDNA, with 17 complete mtDNA sequencing and compared them to each other and to published data from other region of the Indian subcontinent, caste and tribal populations as well as from Iran, Pakistan, Thailand, China, Bangladesh and the Near East.

Our overall analysis of mtDNA variation in KCM population points to a pattern consistent with mixed origins consistent with a location juxtaposed as a middle zone geographically between the Indian subcontinent and the Far East.The haplogroup repertoire present in the KCM data is shaped mainly by the presence of lineages that can be attributed to E-Eurasian, India, (M*, R*). These results trace the sources of mtDNA to India, the Far East, or a mixture of both.

Occurrence of these maternal lineages in the Kuki-Chin-Mizo samples, analysed reveals extensive admixture with local populations, with absence of any remaining possible traces of maternal Near East ancestry. We found no evidence for a maternal Near East origin of contemporary Kuki-Chin-Mizo tribes using this analysis. It is important to note that while we reject maternal Near East ancestry by means of mtDNA analysis, it is possible that for the Y chromosome and autosomal loci different patterns might be observed.