טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
Ph.D Thesis
Ph.D StudentHerman-Bachinsky Yifat
SubjectRegulation of the Drosophila Ubiquitin Ligase DIAP1 is
Mediated via Several Distinct Ubiquitin System
Pathways
DepartmentDepartment of Medicine
Supervisor ? 18? Aaron Ciechanover
Full Thesis textFull thesis text - English Version


Abstract

Inhibitors of apoptosis proteins (IAPs) suppress cell death by inactivating pro-apoptotic regulators, and therefore play important roles in controlling apoptosis in normal and malignant cells.  Many IAPs are ubiquitin ligases, and their activity is mediated via ubiquitination and subsequent degradation of their targets.  Here we corroborate a previous observation that DIAP1 (Drosophila IAP1) can be degraded via a two step mechanism: (i) limited caspase-mediated cleavage, and (ii) degradation of the released fragment via the ubiquitin N-end rule pathway.  Yet, we demonstrate that this pathway is not the only one involved in DIAP1 degradation, and the intact protein can be degraded  independent of prior caspase cleavage.  Importantly, this mode of degradation does not require the RING finger-mediated autoubiquitinating activity of DIAP1 believed to target many RING finger E3s for self destruction.  Our preliminary data suggest that DIAP2 mediates DIAP1 degradation, suggesting a novel regulatory loop within the apoptotic pathway.  Studying the role of the autoubiquitinating activity of DIAP1, we demonstrate that it does not involve formation of Lys48-based polyubiquitin chains, but probably chains linked via lysine 63.  Our preliminary data suggest that autoubiquitination serves to attenuate the ligase activity of DIAP1 towards its exogenous substrates.