M.Sc Thesis

M.Sc StudentNe'eman Nitsa
SubjectBiological Materials in Polymeric Matrices: Multi-
Functional Systems for Applications in Tissue
DepartmentDepartment of Biomedical Engineering
Supervisors PROFESSOR EMERITUS Noah Lotan
MS. Sarit Sivan


Systems operating under a multi-stage regimen usually require that materials be supplied to them under appropriate control, the latter concerning both time and location. Tissue Engineering is a field of activity of particular concern today and involves such complex systems. This activity is aimed at developing functional substitutes for partially or completely damaged tissues and organs.   Accordingly, it relies on the use of biological cells, which grow and multiply on degradable supports (scaffolds) and, in so doing, produce the desired tissue. This highly complex undertaking also requires the supply of appropriate growth factors, each of them at a different stage of the process.

This work presents the development of a novel approach for design and construction of polymer-based three-dimensional scaffolds that contain a few bioactive agents and release each of them according to its own schedule. This technology combines fundamental principles of controlled drug release and tissue engineering and relies on basic physico-chemical characteristics (i.e., dissolution / precipitation) of the polymers involved.  It was implemented in a model system, thus achieving the sequential release of two elements, all-trans retinoic acid and bovine serum albumin (during days and weeks, respectively). The approach and technology involved can be conveniently extended to produce multi-functional modalities.