M.Sc Thesis

M.Sc StudentMorgan Michal
SubjectEffects of GTF Treatment in Preventing Diabetes Mellitus
Damages in Rats and in Vitro
DepartmentDepartment of Medicine
Supervisor MRS Erella Livne (Deceased)


Glucose Tolerance Factors (GTF) is an antidiabetic material extracted from brewer yeasts in our laboratory. The purpose of the present study was to examine the effects of GTF on rat kidney, retina and long bones. Diabetes was induced by a single streptozotocin injection to young male rats. Diabetic animals were fed orally by daily doses of GTF for two weeks. The results indicate that protein levels in urine of diabetic rats were higher compared with control group, and were reduced following preventing treatment with GTF. Nephropathy, including renal and glomerular hypertrophy, messangial expansion with glomerular basement membrane thickening, damaged proximal tubules and glomerular sclerosis, hight e/n Nos immune activity in kidney, high TBARS levels, which ultimately caused progression of proteinuria and renal failure, developed in the kidneys of diabetic rats, was also reduced in the animals treated with GTF. Retinopathy developed in diabetic rats revealed high immune reactivity of GFAP in glial astrocytes layer and in Muller cells, whereas in control and GTF-treated diabetic rats the protein was found only in the glial astrocytes layer. Na/K ATPase activity was reduced also in the photoreceptors of diabetic rats' retina, when compared to the activity in both control and GTF-treated diabetic rats. Changes in tibia revealed reduced width of the epiphyseal growth plate in diabetic rats, as compared with control. Our study shows that GTF derived from yeast is effective in reduction of diabetes-related damages, inhibited the development of nephrophaty and retinophaty, induced in the early stages of diabetes.