Components of Light Microscope, Kohler Illumination. Alignment and Different Contarast Methods. Phase, Dic. Fluorescent Microscope: Excitation and Emission Paths, Fluorescent Filters. Fluorescent Stereo Microscope. Confocal Microscope: Laser Excitation, Pinhole, Detection Path, Optical Sectioning and 3D Reconstruction. Different Detectors: Digital and Ccd Cameras, Pmts. Acquisition/Anlayis Softwares, Signal Digitization and Quantization - Basic Applications, Simple Geometrical Measurements.