Subject: Subject Sylbus: Protein - Dna Interaction and P53 Function - 136090

Protein - Dna Interaction and P53 Function - 136090
Will not be given the year
Credit
Points
2.0
 
  Lecture Exercise Laboratory Project or
Seminar
House
Work
Weekly
Hours
2       2

Determination of the grade according to progress during the semester and the submission of the final thesis


Prerequisites ( Introduction to Biochemistry and Enzymology 134019
and Molecular Biology 134082
and Regulation of Gene Expression 134119 )


Multiple Codes on the Dna Double Helix with Focus on Codes for Packaging Into Nuclesomes and for Recognition by Sequence-Specific Dna Binding Proteins (Dbp). Protein-Dna Interactions - Protein Motifs Involved in Specific Dna Recognition. Protein-Dna Interactions - Direct and Indirect Readout of Dna, the Role of Dna Structure in Specific and Non-Specific Interactions. Methods for Specificity and Selectivity of Interactions in Various Protein-Dna Families. Is There Code for Dna Recognition by Dbp. Target Site Location by Sequence- Specific Dbp. Experimental and Computational Methods to Study Protein- Dna Interactions. Structure of the P53 Protein. Sequence-Specific Binding of P53 to Its Response Elements Regulation of P53 Activity by Post-Translation Modifications. P53 Is a Hub Protein. P53 as a Gene Network. the P53 Circuit Board, P53 as Tumor Suppressor - Response to Cellular Stress. Target Sit Location by P53 Oscillations and Dynamics, P53 Smart Or Dumb. Mutant P53 and Its Oncogenic Activities.

Learning Outcomes
On Successful Completion of This Module, Students Should Be Able to:
1. Understand the Mechanisms Used by Dna-Binding Proteins to Recognize Their Target Sites on Dna.
2. Understand Search Mechanism Used by Dna-Binding Proteins to Locate Their Binding Sites on Dna.
3. Understand How Dna Sequence and Structure Is Used as a Source of Specificity in the Their Binding to Regulaotry Proteins (Transcription Factors, Nucleosome and Architectural Proteins).
4. Understand What We Currenlty Know on P53 as a Transcription Factor That Binds to Multiple Sites on the Genome and Functions in Multiple Regulatory Roles.
5. Understand How the Most Importamnt Hub Protien Works *Current Knowledge) and How It Decide Which Pathway to Activate Upon a Certain Stress.
6. Read the Understand a Current Primary Paper Written on Binding of a Dna Binding Protein to Dna, Or on P53/Dna Interactions, Sufficiently to Present the Subject Orally and Write a Term Paper on It.




Textbooks
PublishedPublisherAuthorsBook
2000university science bookdsv.a. bloomfield, d.m. crothers and v.a. bloomfield, d.m. crothers andstructure, properties and functions.
2004elsevier academic pressc.r. calladine, hr. drew, b.f. luisi and c.r. calladine, hr. drew, b.f. luisi andunderstanding dna

Created in 03/02/2023 Time 05:39:26