טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentNaor Tal
SubjectSynthetic Microbial Communities with Antibiotic-Mediated
Interactions
DepartmentDepartment of Biology
Supervisor Professor Roy Kishony
Full Thesis textFull thesis text - English Version


Abstract

In every ecological systems microbial diversity is observed. Closer look soon will discover that antibiotic producer strains, antibiotic resistant strains and antibiotic sensitive strains are co-exist in the environment. Many earlier studies associate microbial diversity with microbial interaction and the complexity of the microbial community.

The most simple community is a three strains community of Antibiotic Producer-sensitive-resistant strains, which establish an inhibiting “scissors-paper-rock game”. Earlier studies shows that in a community of three species that include one drug production, one strong interaction and two weaker interactions are established.

Computational work predicted that Strong Rock-Paper-Scissors communities can appear by production of multiple drugs, but the main problem is that we lack the experimental tool to study such complex communities. Here we developed experimental tool in order to specifically and efficiently evaluate microbial interaction in multi-drug communities. In order to do so we cloned different types of anti-microbial proteins producer's strains called colicin, which also have been marked fluorescently. We succeeded to use this innovative tool to provide experimental evidence supporting conclusions which until now obtained only by computational studies.

Future studies may benefit from this work and use our new experimental tool in order to answer many questions regarding complex microbial communities with antibiotic mediated interactions.