טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentTomsov Alexey
SubjectUsing Synthetic Biology to Investigate and Utilize the Human
Skin Microbiome
DepartmentDepartment of Biotechnology and Food Engineering
Supervisor Professor Roee Amit
Full Thesis textFull thesis text - English Version


Abstract

In recent years, the importance of the skin microbiome and its effect on a variety of skin conditions was emphasized in the literature.

Skin conditions change the natural skin microbiome; however, the condition-microbiome relationship is not fully understood.

Moreover, little is known about the overall stability of genetically engineered members of the skin microbiome that are introduced into the native population. This research aims to use the latest advances in synthetic biology to establish the therapeutic potential of the engineered microbiome.


A set of assays were developed to identify the necessary parameters for stable incorporation of bacteria that are continuously expressing and secreting a heterologous protein, into the native microbiome.

The ability to efficiently secrete a heterologous therapeutic enzyme (i.e., 3αHSD) in a bacterial member of the scalp microbiome, Bacillus subtilis was demonstrated (US patent application 15/471,194).

The hormone that is believed to be the cause of male pattern hair loss, 5α-DHT, is broken down by the 3α-HSD enzyme.


In addition, the viability, heterologous protein expression and dynamic population effects with the natural biota of the engineered strain was investigated using the developed assays. The results indicate that it may be possible to incorporate within the skin microbiome a natural and continuous secretory apparatus that can potentially aid in the continuous delivery of therapeutic agents for a variety of skin conditions.