טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentBen Bassat Dana
SubjectThe Influence of Flow Intensity and Field Frequency
on Continuous - Flow Dielectrophoretic Trapping
DepartmentDepartment of Mechanical Engineering
Supervisor Professor Gilad Yossifon
Full Thesis textFull thesis text - English Version


Abstract

Dielectrophoresis (DEP), the induced motion of polarizable particles in a nonuniform electric field, is a versatile mechanism to transport, accumulate, separate and characterize micro/nano-scale particles, cells and biomolecules. The integration of DEP systems into microfluidics enables inexpensive, fast, sensitive and label-free detection and analysis of target bio-particles. We examine the combined influence of the intensity of pressure driven background flow and the frequency of the applied field on the continuous-flow dielectrophoretic trapping behavior of micro-particles within a micro-channel. Using an embedded interdigitated electrode array, we find that the measured trapping percentage over a continuous frequency range exhibits several curious effects which are strongly dependent on the flow intensity, including an apparent shift of the crossover frequency and low-frequency dispersion. A numerical and theoretical model accounting for the combined effects of pressure-driven flow, dielectrophoresis and alternating-current electro-osmosis (ACEO) on the equation of motion for the particle is used to qualitatively describe the main experimental results. I will also shortly describe a multi-functional microfluidic platform demonstrating the feasibility of on-chip electroporation integrated with DEP and ACEO assisted cell/particle manipulation into individual microchambers.


Publications:


1.      S. Park, D. Ben-Bassat, G. Yossifon, "Individually Addressable Multi-Chamber Electroporation Platform with Dielectrophoresis and Alternating-Current-Electro-Osmosis Assisted Cell Positioning", Biomicrofluidics, 8 024117-1-15, 2014.


2.      D. Ben-Bassat, A. Boymelgreen, G. Yossifon, "The Influence of Flow Intensity and Field Frequency on Continuous-Flow Dielectrophoretic Trapping", Journal of colloids and interface science, 2015.