טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentSweedan Amro
SubjectSelf Assembly of Single Wall Carbon Nanotube (SWNTs) Based
on Genetically Engineered Fd Filamentous Virus
DepartmentDepartment of Nanoscience and Nanotechnology
Supervisors Professor Yachin Cohen
Professor Sima Yaron


Abstract


Single-walled carbon nanotubes (SWNTs), because of their unique physical, chemical, electronic, and optical properties, are novel exciting nanomaterial and are thus under consideration as a building blocks for advanced nanoscale devices and circuits for diverse applications. Recent efforts focused on the bottom-up assembly of functional nanosystems from nanoscale building blocks have led to substantial advances. However, the difficulties in the precise placement, predicted interaction, design and interconnection of individual building blocks have impeded further progress and efforts directed toward the controlled assembly of functional elements. In this project our effort was focused on design of SWNT based building blocks using designed filamentous virus. We demonstrate directed self-assembly of SWNT utilizing engineered virus as an organizing template. Our SWNT based template offers a unique potential for future applications. The template was obtained by utilizing genome genetic engineered fd filamentous virus as a scaffold for suspending and organizing SWNT. It is anisotropic, self-organizing, with controllable dimension, amenable to practical modification and cheap production. The virus based template was produced by incorporating SWNT-binding peptides into the filament virus, fused to the N-terminal end of the major coat protein. These peptides were selected from combinatorial peptide library by directed evolution methods based on carbon nanotube affinity and specificity. Engineered CNT binding viruses were analyzed by sequencing, biological activity, labeled specific antibody and mass spectrometry. The modified virus proved to be functional, viable and capable of displaying SWNT binding peptides exposed to solution. Imaging by cryo-transmission electron microscopy indicated that the programmable virus was able to bind SWNT forming an ordered composite structure. We expect that the present SWNT-based building blocks can be applied to several applications including nucleation and binding template for nanoparticles, organizing of several functional components, targeting shuttle and nanodevice building blocks. The method described here can also be generally applied to the assembly of anisotropic nanomaterials, organic, biological and inorganic materials for a broad range of applications.