|Ph.D Student||Rotem Asaf|
|Subject||Nuclear Pore Complex Assembly in Vertebrates|
|Department||Department of Biology||Supervisor||Professor Amnon Harel|
|Full Thesis text|
The nuclear envelope of higher eukaryotic cells re-forms at the exit from mitosis, in concert with the assembly of nuclear pore complexes (NPCs). The first step in postmitotic NPC assembly involves the “seeding” of chromatin with ELYS and the Nup107-160 complex. Subsequent steps in the assembly process are poorly understood and different mechanistic models have been proposed to explain the formation of the full supramolecular structure. Here, we show that the initial step of chromatin seeding is negatively regulated by importin beta. Direct imaging of the chromatin attachment sites reveals single sites situated predominantly on the highest substructures of chromatin surface and lacking any sign of annular structures or oligomerized pre-NPCs.
Surprisingly, the inhibition by importin beta is only partially reversed by RanGTP.
Importin beta forms a joint high molecular weight complex in cytosol, containing both ELYS and the Nup107-160 complex. We suggest that initiation sites for NPC assembly contain single copies of chromatin-bound ELYS/Nup107-160 and that the lateral oligomerization of these subunits depends on the recruitment of membrane components. We predict that additional regulators, besides importin beta and Ran, may be involved in coordinating the initial seeding of chromatin with subsequent steps in the NPC assembly pathway.