טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentGoldman Guy
SubjectPrevention of Membrane Biofouling by Use of Bacteriophages
DepartmentDepartment of Civil and Environmental Engineering
Supervisor Professor Robert Armon
Full Thesis text - in Hebrew Full thesis text - Hebrew Version


Abstract

An UF continuous recycled system  was operated for 80 hours with sterile effluents (two types) primarly inoculated with three bacterial species: Pseudomonas aeruginosa, Acinetobacter johnsonii and Bacillus subtilis (separately and combined) and supplemented with their corresponding bacteriophages (phages) versus control (bacteria without phages). The lytic activity of these phages reduced membrane biofouling by an average of  40 to > 60 % compared to control (bacteria only). Phage numbers increased in the concentrate and some were found in the permeate, however no bacteria were found in the permeate. Combinations of one, two and three bacterial species and their specific phages revealed very good inactivation rates as well reduced biofouling as observed under high resolution electron scaninng microscope (HSEM). The advantage of phage utilization in combating biofouling in such system as UF was demonstrated, pointing towards future application in other membrane processes such as: nanofiltration and reverse osmosis.