טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentGantman Marina
SubjectMapping of Sites within the Tetrahymena Telomerase
Catalytic Subunit that Interact with DNA Primers
in Telomerase Elongation Complexes
DepartmentDepartment of Biology
Supervisors Professor Haim Manor
Professor Emeritus Dan Cassel
Full Thesis text - in Hebrew Full thesis text - Hebrew Version


Abstract

Telomeres are DNA-protein complexes found at the ends o linier eukaryotic chromosomes. These complexes protect the chromosomes from degradation and fusion with other chromosomes. Telomerase is a reverse transcriptase that uses an integral RNA molecule as a template for elongating the telomeric DNA. In addition to the RNA subunit, the telomerase core enzyme contains a protein subunit designated  TERT. My research focused on mapping the interactions of the telomeric DNA with TERT in an active enzyme. My study revealed a new interaction with the amino acid residue phenyl alanine #192 in TERT.