טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentRaed Khamaisi
SubjectMicrochannel Flow Device for the Study of Microcirculatory
Blood Flow
DepartmentDepartment of Biomedical Engineering
Supervisors Professor Emeritus Dinnar Uri (Deceased)
Professor Emeritus Nemirovsky Yael
Full Thesis textFull thesis text - English Version


Abstract

Today there is growing interest in research on microfluidic systems. One of the basic components in microfluidic systems is microchannels.

There are many medical and biological applications for Microfluidics devices such as cell manipulation, sorters and diagnostic instruments.

 To build these devices it is necessary to have an understanding of how the complex biological fluids behave inside small structures. The importance of the microcirculation is highlighted by the fact that most of the hydrodynamic resistance of the circulatory system lies in the microvessels.

The research goal is to better understand the Microcirculatory blood flow and the study of biomedical parameters of Microcirculatory. These parameters are needed to investigate the micro- level and to predict the flow behavior. The main interest in microcirculation includes pressure-flow relationship.

In the first part of our research we developed and fabricated a Lap-Biochip. This Lap-Biochip accounted for the most essential microstructural and geometrical features of Microcirculatory. This enabled the possibility of using Lap-Biochip to investigate the Microcirculatory parameters.

The Lap-Biochip based microchannel was designed and fabricated by using standard planar photolithography process on a 300μm silicon substrate.

In the experimental part of this work, an apparatus was constructed and a procedure devised to measure the volume flow rate Q and the pressure drop across the microchannel ΔP. The test modules used straight microchannels, bifurcation microchannels and looped microchannels to study the differences in behaviors of blood and biological fluids.

The results indicated that the fabrication of the microchannel into rigid silicon offered excellent control for studying microcirculation on network microchannels.

The experimental result shows that the flow in microchannels behaves similar to the conventional size channels and the regular method used to evaluate the flow is satisfactory. The theoretical curves of the flow rate, as a function of the pressure drop, are all linear. The experimental curves of the flow rate were also linear, as required by conventional laminar flow theory.

In summary it can be said that the present research proves that the flow maps several types of microchannels.

Understanding the flow makes it possible to develop and build Microfluidic devices for medical and biological applications.

This system could extend to other application. First the system can provide observation of cellular response to the endothelial lining of a vessel. Second, the system allows observation of blood cell flow through an extensive network of microvessels similar to those found in the microcirculation.