טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentEliaz-Volkovich Anat
SubjectSimulation of Diabetic Conditions on the Eye Lens in Organ
Culture: Mechanisms Leading to Damage (Cataract)
and its Prevention
DepartmentDepartment of Medicine
Supervisors Dr. Ahuva Dovrat
Dr. Nitsa Mirsky


Abstract

Cataract is defined as an opacification of the lens. Cataract develops earlier in patients with diabetes than in those without diabetes. The aim of the research was to investigate the mechanisms involved in diabetic cataractogenesis, and the effects of GTF (an anti-diabetic agent), in preventing glucose damage to the eye lens. Using an in-vitro system, we examined the effects of 450mg% glucose (that induces acute diabetes) and GTF supplement, on bovine eye lenses, during 14 days in organ culture. We followed changes in lens optical quality throughout the culture period. At the end of the culture lenses were observed by inverted microscopy and epithelial samples taken for enzyme analysis. High levels of glucose caused optical damage to bovine lenses in culture. GTF reduced the damage caused by glucose. The activities of Na+, K+, ATPase were reduced in the presence of glucose. GTF attenuated the Na+, K+, ATPase damage. microscopic analysis showed induction of  vesicles in lens epithelium and cortex as evidence of damage to the lens caused by glucose, which was reduced by GTF. Bovine lens proteins, the crystallins, also showed changes in the presence of glucose. The use of specific antibodies against lens proteins helped us determine which proteins were damaged by exposure to glucose. Our results indicate. GTF reduced the damage caused by high glucose levels to bovine lens.