טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentDoron Maayan
SubjectPhenotypic, Genetic and Molecular Characterization of
Disintegrin-Metalloproteases Involved in Cell
Migration in Caenorhabditis elegans
DepartmentDepartment of Biology
Supervisor Professor Benjamin Podbilewicz


Abstract

The ADAM protein family is comprised of members that share structural characteristics. ADAMs are involved in various developmental processes. In this work we studied two of the ADAM members in the free-living nematode Caenorhabditis elegans: adm-1 and adm-2.

adm-1 was cloned and characterized by Benjamin Podbilewicz (Podbilewicz, 1996).  Several polyclonal antibodies were raised against some peptides of the protein. In a later study it was discovered that adm-1 and unc-71 are allelic  (Huang et al., 2003).  We used our antibodies in a set of biochemical trials in order to determine where the ADM-1 protein is expressed in the nematode, and characterized defects in the axon guidance process of motor-neurons.  We found that there is a crossreacting endogenous protein that is specifically recognized by our antibodies, thus, we could not conclude where exactly the protein is expressed. adm-1/unc-71 participates in the process of axon guidance and sex myoblast migration.

adm-2 was cloned and characterized in our lab. In this study we wanted to determine the characteristics and function of this gene in C. elegans.  We analyzed a deletion mutant and RNAi feeding method to interfere with gene expression, and found that the gene is not essential for embryonic development. We further analyzed strains that exhibit GFP construct of the transcript and protein.  We found that the transcript is expressed differently than the translated protein, but both are expressed in neurons which share some features, and apparently participate in the worm’s reaction to external stimuli. We found that adm-2 participates in the proper pathfinding of some motorneurons axons.