טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentHava Segal
SubjectIdentification of New Antigens Specific for Small Cell Lung
Cancer and Development of a Diagnostic Kit Based
on Serologic Response
DepartmentDepartment of Biology
Supervisor Full Professor Admon Arie


Abstract

Small Cell Lung Cancer (SCLC) is an aggressive type of lung cancer with metastasis present usually at early stages and as in other cancers early detection can improve prognosis. This research aims to identify proteins against which antibodies are present in the serum of the cancer patients. Such proteins can be used to develop new diagnostic tools for early detection of the disease or for immunotherapy.

            The research was based on Serological Proteomics. The protein content of SCLC cells extracts are resolved by running multiple two dimensional gel electrophoresis in parallel, followed by Coomassie staining of one of the gels and Western blotting of the rest. The blots were probed with serum antibodies of cancer patients or serum antibodies of healthy individuals (controls). Proteins spots exclusively lighted by serum antibodies of cancer patients were excised from the Coomassie stained gel. The proteins in these spots were digested with trypsin and identified by tandem mass spectrometry. 

            Using this serological proteomics approach we identify a number of proteins against which antibodies are exclusively present in the serum of the SCLC cancer patients. These include proteins with different cellular functions, normally present in different sub-cellular locations. The genes encoding the some of the serologically active proteins were cloned and test of their proteins products were began as markers for early detection of the disease. These proteins will also be evaluated in the future for their potential as cancer vaccines by testing their ability to elicit cellular immune response with patient's lymphocytes.