טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentBerman Rotem Sara
SubjectSequence Specific Patterning of DNA-Templated Electronics
DepartmentDepartment of Physics
Supervisors Professor Erez Braun
Professor Uri Sivan


Abstract

Molecular electronics deals with the electronic properties of molecules and their incorporation into electronic devices and circuits. A promising concept is the use of self assembly based on molecular recognition to construct electronic circuits. This thesis presents advances in the employment of biological molecules to form self assembled electronic circuits. 

Our group has developed tools to advance the use of DNA molecules to direct the self assembly of molecular electronics. A way to create DNA templated gold wires has been developed, followed by a "molecular lithography" method, which relies on homologous genetic-recombination processes carried out by the RecA protein from Escherichia-coli bacteria. In a sequence-specific manner, it enables the patterning of DNA metal coating, the localization of molecular objects on specific sites along the DNA substrate, and the generation of molecularly accurate stable DNA junctions. This thesis presents the use of molecular lithography to create the first entirely self-assembled electronic device, a DNA-templated carbon nanotube field-effect transistor.

A unique contribution of this thesis is the development of an improved molecular lithography method, which enables subsequent manipulations of patterned molecules. The improved method uses the patterning of a reducing agent binding to control the metallization pattern. It enables the creation of biologically reactive DNA molecules with embedded metallization pattern, which can be revealed after the assembly of elaborated DNA constructs. This achievement opens the way to the assembly of complicated DNA structures with electronic functionality and advance the ability to self assemble molecular objects into electronic circuits.