|M.Sc Student||Einat Souli|
|Subject||Effect of Indole-3-Carbinol (I3C) on Prostate|
Cancer Cells in Vitro and in Vivo
|Department||Department of Biotechnology and Food Engineering||Supervisor||Professor Emeritus Yannai Shmuel (Deceased)|
extensive and consistent evidence that high intake of cruciferous vegetables (e.g.,
cabbage, broccoli, cauliflower) are involved in cancer preventation in vitro
and in vivo. These vegetables contain glucobrassicin, an indolylmethyl
glucosinolate, which is metabolised into several compounds. When the plant
cells are damaged by cutting or chewing, the enzyme myrosinase cleaves
glucobrassicin into indole-3-carbinol (I3C). In acidic pH, I3C condenses to
form 3,3'-diindolymethane (DIM). In this study, we examined the effect of I3C
on normal cells and prostate cancer cell lines. We observed that I3C has a
significant inhibitory effect on the viability of all prostate cancer cell
types in a time- and dose-dependent manner, and is accompanied by inhibition of
DNA synthesis and apoptosis. Normal cell types were less influenced. The
anti-carcinogenic effects of I3C as preventive and therapeutic treatment were
examined in mice. The findings indicate that in both cases the treatment with
I3C caused a significant decrease in tumour volume, compared with the controls.
A significant decrease in cell proliferation and angiogenesis, and increase in
apoptosis were observed in tumours from mice treated with I3C. I3C did not
affect body weight, or kidney and live function, in any of the mice groups.
Thus, using I3C at the concentration tested seems to be safe, with no toxic
effects to be expected.
It seems that consumption of larger daily amounts of cruciferous vegetables, or taking encapsulated I3C-containing pills, can be a feasible approach to prostate cancer chemoprevention and appears to constitute an effective therapeutic treatment as well.