טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentSouli Einat
SubjectEffect of Indole-3-Carbinol (I3C) on Prostate
Cancer Cells in Vitro and in Vivo
DepartmentDepartment of Biotechnology and Food Engineering
Supervisor Professor Emeritus Shmuel Yannai (Deceased)


Abstract

There is extensive and consistent evidence that high intake of cruciferous vegetables (e.g., cabbage, broccoli, cauliflower) are involved in cancer preventation in vitro and in vivo. These vegetables contain glucobrassicin, an indolylmethyl glucosinolate, which is metabolised into several compounds. When the plant cells are damaged by cutting or chewing, the enzyme myrosinase cleaves glucobrassicin into indole-3-carbinol (I3C). In acidic pH, I3C condenses to form 3,3'-diindolymethane (DIM). In this study, we examined the effect of I3C on normal cells and prostate cancer cell lines. We observed that I3C has a significant inhibitory effect on the viability of all prostate cancer cell types in a time- and dose-dependent manner, and is accompanied by inhibition of DNA synthesis and apoptosis. Normal cell types were less influenced. The anti-carcinogenic effects of I3C as preventive and therapeutic treatment were examined in mice. The findings indicate that in both cases the treatment with I3C caused a significant decrease in tumour volume, compared with the controls. A significant decrease in cell proliferation and angiogenesis, and increase in apoptosis were observed in tumours from mice treated with I3C. I3C did not affect body weight, or kidney and live function, in any of the mice groups. Thus, using I3C at the concentration tested seems to be safe, with no toxic effects to be expected.
It seems that consumption of larger daily amounts of cruciferous vegetables, or taking encapsulated I3C-containing pills, can be a feasible approach to prostate cancer chemoprevention and appears to constitute an effective therapeutic treatment as well.