טכניון מכון טכנולוגי לישראל
הטכניון מכון טכנולוגי לישראל - בית הספר ללימודי מוסמכים  
M.Sc Thesis
M.Sc StudentMichael Inbal
SubjectMolecular Nanocapsules for Protection and Controlled Release
of Anticarcinogenic Fatty Acid
DepartmentDepartment of Biotechnology and Food Engineering
Supervisor Dr. Eyal Shimoni


Abstract

Polyunsaturated fatty acids (PUFA) play major roles in health and disease. In order to enrich foods with PUFA, a delivery system must be used. The goal of this study was to develop efficient delivery system for PUFA that will deliver them to specific sites in the digestive tract, and will also provide them with adequate protection from oxygen and heat. Nanocapsules, which are inclusion complexes created by using molecular interactions of amylose with lipids, serve as the delivery system. Conjugated linoleic acid (CLA) was used to create the amylose-lipid delivery system.  Complexes of amylose-CLA were created under different conditions: method, temperature and degree of polymerization. These complexes were characterized by x-ray diffraction (XRD), differential scanning calorimetry (DSC) and atomic force microscopy (AFM). The stability from oxidation afforded to CLA by complexation, and the complexes dissolution in simulated stomach conditions and enzymatic digestion were also examined. The results of the presented research show that amylose can complex with CLA. The complexes formed provide stability to oxidation and thermal treatments, such as pasteurisation.  Control of CLA release is enabled, and the CLA release does not occur in simulated stomach conditions, rather it is driven by amylolytic activity of pancreatin, which indicates that the location of release in the digestive tract will probably be in the intestine. Overall, the results indicate that the amylose-lipid complex system can serve as a vehicle for delivery of PUFA to the intestine.